8TCF

Integrin alpha-v beta-8 in complex with minibinder B8_BP_dsulf

Summary for 8TCF

• Entry DOI: 10.2210/pdb8tcf/pdb
• EMDB information: 41153
• Descriptor: Integrin alpha-V heavy chain, Integrin beta-8, Minibinder B8_BP_dslf, ... (8 entities in total)
• Functional Keywords: complex, heterodimer, signaling, tgf-beta, de novo protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 100616.99

Authors

Campbell, M.G.,Fernandez, A.,Roy, A.,Kraft, J.,Baker, D. (deposition date: 2023-06-30, release date: 2023-09-27, Last modification date: 2024-11-06)

Primary citation

Roy, A.,Shi, L.,Chang, A.,Dong, X.,Fernandez, A.,Kraft, J.C.,Li, J.,Le, V.Q.,Winegar, R.V.,Cherf, G.M.,Slocum, D.,Poulson, P.D.,Casper, G.E.,Vallecillo-Zuniga, M.L.,Valdoz, J.C.,Miranda, M.C.,Bai, H.,Kipnis, Y.,Olshefsky, A.,Priya, T.,Carter, L.,Ravichandran, R.,Chow, C.M.,Johnson, M.R.,Cheng, S.,Smith, M.,Overed-Sayer, C.,Finch, D.K.,Lowe, D.,Bera, A.K.,Matute-Bello, G.,Birkland, T.P.,DiMaio, F.,Raghu, G.,Cochran, J.R.,Stewart, L.J.,Campbell, M.G.,Van Ry, P.M.,Springer, T.,Baker, D.
De novo design of highly selective miniprotein inhibitors of integrins alpha v beta 6 and alpha v beta 8.
Nat Commun, 14:5660-5660, 2023

PubMed Abstract: The RGD (Arg-Gly-Asp)-binding integrins αvβ6 and αvβ8 are clinically validated cancer and fibrosis targets of considerable therapeutic importance. Compounds that can discriminate between homologous αvβ6 and αvβ8 and other RGD integrins, stabilize specific conformational states, and have high thermal stability could have considerable therapeutic utility. Existing small molecule and antibody inhibitors do not have all these properties, and hence new approaches are needed. Here we describe a generalized method for computationally designing RGD-containing miniproteins selective for a single RGD integrin heterodimer and conformational state. We design hyperstable, selective αvβ6 and αvβ8 inhibitors that bind with picomolar affinity. CryoEM structures of the designed inhibitor-integrin complexes are very close to the computational design models, and show that the inhibitors stabilize specific conformational states of the αvβ6 and the αvβ8 integrins. In a lung fibrosis mouse model, the αvβ6 inhibitor potently reduced fibrotic burden and improved overall lung mechanics, demonstrating the therapeutic potential of de novo designed integrin binding proteins with high selectivity.

PubMed: 37704610
DOI: 10.1038/s41467-023-41272-z

Experimental method

ELECTRON MICROSCOPY (2.9 Å)