8T9W
Crystal structure of a GH140 apiosidase derived from a lignocellulolytic enriched mangrove metagenome.
Summary for 8T9W
| Entry DOI | 10.2210/pdb8t9w/pdb |
| Descriptor | Apiosidase (2 entities in total) |
| Functional Keywords | metagenome, glycosyl hydrolase family 140, apiose, endo-apiosidase, hydrolase |
| Biological source | metagenome |
| Total number of polymer chains | 4 |
| Total formula weight | 198358.03 |
| Authors | Liberato, M.V.,Squina, F. (deposition date: 2023-06-26, release date: 2024-02-07, Last modification date: 2024-11-13) |
| Primary citation | Liberato, M.V.,Paixao, D.A.A.,Tomazetto, G.,Ndeh, D.,Bolam, D.N.,Squina, F.M. Discovery, structural characterization, and functional insights into a novel apiosidase from the GH140 family, isolated from a lignocellulolytic-enriched mangrove microbial community. Biotechnol.Lett., 46:201-211, 2024 Cited by PubMed Abstract: Apiosidases are enzymes that cleave the glycosidic bond between the monosaccharides linked to apiose, a branched chain furanose found in the cell walls of vascular plants and aquatic monocots. There is biotechnological interest in this enzyme group because apiose is the flavor-active compound of grapes, fruit juice, and wine, and the monosaccharide is found to be a plant secondary metabolite with pharmaceutical properties. However, functional and structural studies of this enzyme family are scarce. Recently, a glycoside hydrolase family member GH140 was isolated from Bacteroides thetaiotaomicron and identified as an endo-apiosidase. PubMed: 38280177DOI: 10.1007/s10529-023-03460-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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