8T9W
Crystal structure of a GH140 apiosidase derived from a lignocellulolytic enriched mangrove metagenome.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.093, 113.391, 158.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.330 - 2.200 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.567 |
| Data reduction software | XDS (1.13) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.190 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.195 | 1.054 |
| Rmeas | 0.213 | 1.165 |
| Rpim | 0.084 | 0.486 |
| Total number of observations | 655800 | 28473 |
| Number of reflections | 102431 | 5019 |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.4 | 5.7 |
| CC(1/2) | 0.991 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 12,5 % PEG8000, 0,1 M Hepes |
