8T3V
Cryo-EM structure of the DHA bound FFA1-Gq complex
Summary for 8T3V
| Entry DOI | 10.2210/pdb8t3v/pdb |
| EMDB information | 41013 |
| Descriptor | Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (7 entities in total) |
| Functional Keywords | gpcr, agonist, membrane protein |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 5 |
| Total formula weight | 129092.80 |
| Authors | Zhang, X.,Tikhonova, I.,Milligan, G.,Zhang, C. (deposition date: 2023-06-07, release date: 2024-01-24, Last modification date: 2024-11-06) |
| Primary citation | Zhang, X.,Guseinov, A.A.,Jenkins, L.,Li, K.,Tikhonova, I.G.,Milligan, G.,Zhang, C. Structural basis for the ligand recognition and signaling of free fatty acid receptors. Sci Adv, 10:eadj2384-eadj2384, 2024 Cited by PubMed Abstract: Free fatty acid receptors 1 to 4 (FFA1 to FFA4) are class A G protein-coupled receptors (GPCRs). FFA1 to FFA3 share substantial sequence similarity, whereas FFA4 is unrelated. However, FFA1 and FFA4 are activated by long-chain fatty acids, while FFA2 and FFA3 respond to short-chain fatty acids generated by intestinal microbiota. FFA1, FFA2, and FFA4 are potential drug targets for metabolic and inflammatory conditions. Here, we determined the active structures of FFA1 and FFA4 bound to docosahexaenoic acid, FFA4 bound to the synthetic agonist TUG-891, and butyrate-bound FFA2, each complexed with an engineered heterotrimeric G protein (miniG), by cryo-electron microscopy. Together with computational simulations and mutagenesis studies, we elucidated the similarities and differences in the binding modes of fatty acid ligands to their respective GPCRs. Our findings unveiled distinct mechanisms of receptor activation and G protein coupling. We anticipate that these outcomes will facilitate structure-based drug development and underpin future research on this group of GPCRs. PubMed: 38198545DOI: 10.1126/sciadv.adj2384 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.39 Å) |
Structure validation
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