8SZ8
Cryo-EM of the GDP-bound human dynamin polymer assembled on the membrane in the super constricted state (full helix)
This is a non-PDB format compatible entry.
Summary for 8SZ8
| Entry DOI | 10.2210/pdb8sz8/pdb |
| Related | 8SXZ |
| EMDB information | 40861 40903 |
| Descriptor | Dynamin-1, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total) |
| Functional Keywords | dynamin, membrane, fission, lipid, tubule, scission, endocytosis, hydrolase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 4 |
| Total formula weight | 347120.52 |
| Authors | Jimah, J.R.,Canagarajah, B.J.,Hinshaw, J.E. (deposition date: 2023-05-27, release date: 2024-05-01, Last modification date: 2024-08-07) |
| Primary citation | Jimah, J.R.,Kundu, N.,Stanton, A.E.,Sochacki, K.A.,Canagarajah, B.,Chan, L.,Strub, M.P.,Wang, H.,Taraska, J.W.,Hinshaw, J.E. Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission. Dev.Cell, 59:1783-, 2024 Cited by PubMed Abstract: Dynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the plasma membrane. Although atomic models of the dynamin helical polymer bound to guanosine triphosphate (GTP) analogs define earlier stages of membrane constriction, there are no atomic models of the assembled state post-GTP hydrolysis. Here, we used cryo-EM methods to determine atomic structures of the dynamin helical polymer assembled on lipid tubules, akin to necks of budding endocytic vesicles, in a guanosine diphosphate (GDP)-bound, super-constricted state. In this state, dynamin is assembled as a 2-start helix with an inner lumen of 3.4 nm, primed for spontaneous fission. Additionally, by cryo-electron tomography, we trapped dynamin helical assemblies within HeLa cells using the GTPase-defective dynamin K44A mutant and observed diverse dynamin helices, demonstrating that dynamin can accommodate a range of assembled complexes in cells that likely precede membrane fission. PubMed: 38663399DOI: 10.1016/j.devcel.2024.04.008 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.55 Å) |
Structure validation
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