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8SY5

E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dS-BTP base pair in the active site

Summary for 8SY5
Entry DOI10.2210/pdb8sy5/pdb
EMDB information40862
DescriptorDNA-directed RNA polymerase subunit alpha, 2-oxo-2-hydroadenosine 5'-(tetrahydrogen triphosphate), DNA-directed RNA polymerase subunit beta, ... (11 entities in total)
Functional Keywordscomplex, unnatural base pairs, synthetic biology, transcription, transcription-dna-rna complex, transcription/dna/rna
Biological sourceEscherichia coli
More
Total number of polymer chains8
Total formula weight410283.88
Authors
Shan, Z.,Lyumkis, D.,Oh, J.,Wang, D. (deposition date: 2023-05-24, release date: 2023-12-27)
Primary citationOh, J.,Shan, Z.,Hoshika, S.,Xu, J.,Chong, J.,Benner, S.A.,Lyumkis, D.,Wang, D.
A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase.
Nat Commun, 14:8219-8219, 2023
Cited by
PubMed Abstract: Artificially Expanded Genetic Information Systems (AEGIS) add independently replicable unnatural nucleotide pairs to the natural G:C and A:T/U pairs found in native DNA, joining the unnatural pairs through alternative modes of hydrogen bonding. Whether and how AEGIS pairs are recognized and processed by multi-subunit cellular RNA polymerases (RNAPs) remains unknown. Here, we show that E. coli RNAP selectively recognizes unnatural nucleobases in a six-letter expanded genetic system. High-resolution cryo-EM structures of three RNAP elongation complexes containing template-substrate UBPs reveal the shared principles behind the recognition of AEGIS and natural base pairs. In these structures, RNAPs are captured in an active state, poised to perform the chemistry step. At this point, the unnatural base pair adopts a Watson-Crick geometry, and the trigger loop is folded into an active conformation, indicating that the mechanistic principles underlying recognition and incorporation of natural base pairs also apply to AEGIS unnatural base pairs. These data validate the design philosophy of AEGIS unnatural basepairs. Further, we provide structural evidence supporting a long-standing hypothesis that pair mismatch during transcription occurs via tautomerization. Together, our work highlights the importance of Watson-Crick complementarity underlying the design principles of AEGIS base pair recognition.
PubMed: 38086811
DOI: 10.1038/s41467-023-43735-9
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.7 Å)
Structure validation

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