8SY5
E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dS-BTP base pair in the active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, G | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | I | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | J | DNA-directed RNA polymerase subunit beta' | polymer | 1430 | 158105.7 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | K | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | N | Non-template single stranded DNA | polymer | 18 | 5663.7 | 1 | synthetic construct | ||
6 | R | RNA oligomer | polymer | 9 | 2934.8 | 1 | synthetic construct | ||
7 | T | Template single stranded DNA | polymer | 29 | 8713.6 | 1 | synthetic construct | ||
8 | J | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
9 | J | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
10 | J | 2-oxo-2-hydroadenosine 5'-(tetrahydrogen triphosphate) | non-polymer | 523.2 | 1 | Chemie (X0F) | |||
11 | water | water | 18.0 | 375 | Chemie (HOH) |
Sequence modifications
J: 1 - 1407 (UniProt: P0A8T7)
PDB | External Database | Details |
---|---|---|
Leu 1408 | - | expression tag |
Glu 1409 | - | expression tag |
Leu 1410 | - | expression tag |
Glu 1411 | - | expression tag |
Val 1412 | - | expression tag |
Leu 1413 | - | expression tag |
Phe 1414 | - | expression tag |
Gln 1415 | - | expression tag |
Gly 1416 | - | expression tag |
Pro 1417 | - | expression tag |
Ser 1418 | - | expression tag |
Ser 1419 | - | expression tag |
Gly 1420 | - | expression tag |
His 1421 | - | expression tag |
His 1422 | - | expression tag |
His 1423 | - | expression tag |
His 1424 | - | expression tag |
His 1425 | - | expression tag |
His 1426 | - | expression tag |
His 1427 | - | expression tag |
His 1428 | - | expression tag |
His 1429 | - | expression tag |
His 1430 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 409605.6 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 678.3 | |
All* | Total formula weight | 410283.9 |