8SV8

Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex from a composite map

Summary for 8SV8

Entry DOI	10.2210/pdb8sv8/pdb
EMDB information	40407 40408 40782 40799
Descriptor	Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-like protein ISG15, Ubiquitin/ISG15-conjugating enzyme E2 L6, ... (4 entities in total)
Functional Keywords	complex, ubiquitin, ligase, signaling protein
Biological source	Homo sapiens (human) More
Total number of polymer chains	4
Total formula weight	164107.97
Authors	Afsar, M.,Jia, L.,Ruben, E.A.,Olsen, S.K. (deposition date: 2023-05-15, release date: 2023-10-11)
Primary citation	Afsar, M.,Liu, G.,Jia, L.,Ruben, E.A.,Nayak, D.,Sayyad, Z.,Bury, P.D.S.,Cano, K.E.,Nayak, A.,Zhao, X.R.,Shukla, A.,Sung, P.,Wasmuth, E.V.,Gack, M.U.,Olsen, S.K. Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. Nat Commun, 14:4786-4786, 2023 Cited by PubMed: 37553340 DOI: 10.1038/s41467-023-39780-z PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.38 Å)

Structure validation