8SV8

Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex from a composite map

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-like modifier-activating enzyme 7	polymer	1012	111822.1	1	UniProt (P41226) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) In PDB	Homo sapiens (human)	Ubiquitin-activating enzyme 7,D8,Ubiquitin-activating enzyme E1 homolog
2	B, D	Ubiquitin-like protein ISG15	polymer	157	17147.6	2	UniProt (P05161) Pfam (PF00240) In PDB	Homo sapiens (human)	Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein,hUCRP
3	C	Ubiquitin/ISG15-conjugating enzyme E2 L6	polymer	152	17643.4	1	UniProt (O14933) Pfam (PF00179) In PDB	Homo sapiens (human)	E2 ubiquitin-conjugating enzyme L6,Retinoic acid-induced gene B protein,RIG-B,UbcH8,Ubiquitin carrier protein L6,Ubiquitin-protein ligase L6
4	B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

B, D: 1 - 157 (UniProt: P05161)

PDB	External Database	Details
Ser 78	Cys 78	engineered mutation

C: 2 - 153 (UniProt: O14933)

PDB	External Database	Details
Ser 98	Cys 98	engineered mutation
Ser 102	Cys 102	engineered mutation
Lys 121	Leu 121	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	163760.8
Non-Polymers*	Number of molecules	1
	Total formula weight	347.2
All*	Total formula weight	164108.0

*Water molecules are not included.