8SF8

Structure of bovine PKA bound to (R)-N-(4-(1H-pyrrolo[2,3-b]pyridin-4-yl)phenyl)-2-amino-4-methylpentanamide

Summary for 8SF8

• Entry DOI: 10.2210/pdb8sf8/pdb
• Descriptor: cAMP-dependent protein kinase catalytic subunit alpha, cAMP-dependent protein kinase inhibitor alpha, N-[4-(1H-pyrrolo[2,3-b]pyridin-4-yl)phenyl]-D-leucinamide, ... (4 entities in total)
• Functional Keywords: kinase, type i kinase inhibitor, co-crystal structure, cytosolic protein
• Biological source: Bos taurus (cattle); More
• Total number of polymer chains: 2
• Total formula weight: 43661.60

Authors

Coker, J.A.,Arya, T.,Goins, C.M.,Maw, J.J.,Macdonald, J.D.,Stauffer, S.R. (deposition date: 2023-04-10, release date: 2024-02-21, Last modification date: 2024-03-06)

Primary citation

Maw, J.J.,Coker, J.A.,Arya, T.,Goins, C.M.,Sonawane, D.,Han, S.H.,Rees, M.G.,Ronan, M.M.,Roth, J.A.,Wang, N.S.,Heemers, H.V.,Macdonald, J.D.,Stauffer, S.R.
Discovery and Characterization of Selective, First-in-Class Inhibitors of Citron Kinase.
J.Med.Chem., 67:2631-2666, 2024

PubMed Abstract: Citron kinase (CITK) is an AGC-family serine/threonine kinase that regulates cytokinesis. Despite knockdown experiments implicating CITK as an anticancer target, no selective CITK inhibitors exist. We transformed a previously reported kinase inhibitor with weak off-target CITK activity into a first-in-class CITK chemical probe, . is a Type I kinase inhibitor which potently inhibits CITK catalytic activity (biochemical IC = 12 nM), binds directly to full-length human CITK in cells (NanoBRET < 10 nM), and demonstrates favorable DMPK properties for in vivo evaluation. We engineered exquisite selectivity for CITK (>17-fold versus 373 other human kinases), making the first chemical probe suitable for interrogating the complex biology of CITK. Our small-molecule CITK inhibitors could not phenocopy the effects of CITK knockdown in cell proliferation, cell cycle progression, or cytokinesis assays, providing preliminary evidence that the structural roles of CITK may be more important than its kinase activity.

PubMed: 38330278
DOI: 10.1021/acs.jmedchem.3c01807

Experimental method

X-RAY DIFFRACTION (1.7 Å)