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8SCB

Terminating ribosome with SRI-41315

This is a non-PDB format compatible entry.
Summary for 8SCB
Entry DOI10.2210/pdb8scb/pdb
EMDB information40344
DescriptorRibosomal protein L8, 60S ribosomal protein L11, eL13, ... (92 entities in total)
Functional Keywordstermination, sri-41315, ribosome
Biological sourceHomo sapiens
More
Total number of polymer chains87
Total formula weight3615287.80
Authors
Yip, M.C.J.,Coelho, J.P.L.,Oltion, K.,Tauton, J.,Shao, S. (deposition date: 2023-04-05, release date: 2023-12-27, Last modification date: 2024-10-09)
Primary citationCoelho, J.P.L.,Yip, M.C.J.,Oltion, K.,Taunton, J.,Shao, S.
The eRF1 degrader SRI-41315 acts as a molecular glue at the ribosomal decoding center.
Nat.Chem.Biol., 20:877-884, 2024
Cited by
PubMed Abstract: Translation termination is an essential cellular process, which is also of therapeutic interest for diseases that manifest from premature stop codons. In eukaryotes, translation termination requires eRF1, which recognizes stop codons, catalyzes the release of nascent proteins from ribosomes and facilitates ribosome recycling. The small molecule SRI-41315 triggers eRF1 degradation and enhances translational readthrough of premature stop codons. However, the mechanism of action of SRI-41315 on eRF1 and translation is not known. Here we report cryo-EM structures showing that SRI-41315 acts as a metal-dependent molecular glue between the N domain of eRF1 responsible for stop codon recognition and the ribosomal subunit interface near the decoding center. Retention of eRF1 on ribosomes by SRI-41315 leads to ribosome collisions, eRF1 ubiquitylation and a higher frequency of translation termination at near-cognate stop codons. Our findings reveal a new mechanism of release factor inhibition and additional implications for pharmacologically targeting eRF1.
PubMed: 38172604
DOI: 10.1038/s41589-023-01521-0
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.5 Å)
Structure validation

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