8RYH

Nitratidesulfovibrio vulgaris [FeFe]-hydrogenase [FeFe]-hydrogenase variant with both subunits linked by a linker peptide derived from a group A1 type [FeFe]-hydrogenase of Veillonella atypica

Summary for 8RYH

Entry DOI	10.2210/pdb8ryh/pdb
Descriptor	Periplasmic [Fe] hydrogenase large subunit,Periplasmic [Fe] hydrogenase small subunit, IRON/SULFUR CLUSTER, dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+), ... (4 entities in total)
Functional Keywords	[fefe] hydrogenase, iron-sulfur cluster, metalloenzyme, hydrogen production, fusion protein, oxidoreductase
Biological source	Nitratidesulfovibrio vulgaris More
Total number of polymer chains	1
Total formula weight	55466.83
Authors	Bikbaev, K.,Jaenecke, J.,Winkler, M.,Span, I. (deposition date: 2024-02-08, release date: 2025-02-19, Last modification date: 2026-02-25)
Primary citation	Winkler, M.H.,Jaenecke, J.,Bikbaev, K.,Bronold, J.,Yadav, S.,Apfel, U.P.,Birrell, J.A.,Span, I.,Plumere, N.,Leger, C.,Malagnini, M. Subunit fusion unlocks rapid in vitro maturation for slowly activating heterodimeric [FeFe]-hydrogenases Chem Sci, 2026 Cited by DOI: 10.1039/D5SC07299A PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.773 Å)

Structure validation