8RRH

The human prohibitin complex

Summary for 8RRH

• Entry DOI: 10.2210/pdb8rrh/pdb
• EMDB information: 19459
• Descriptor: Prohibitin 1, Prohibitin-2 (2 entities in total)
• Functional Keywords: chaperone, lipid organization, protease regulator, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 11
• Total formula weight: 345734.95

Authors

Lange, F.,Ratz, M.,Dohrke, J.N.,Wenzel, D.,Riedel, D.,Ilgen, P.,Jakobs, S. (deposition date: 2024-01-22, release date: 2024-12-18, Last modification date: 2025-04-02)

Primary citation

Lange, F.,Ratz, M.,Dohrke, J.N.,Le Vasseur, M.,Wenzel, D.,Ilgen, P.,Riedel, D.,Jakobs, S.
In situ architecture of the human prohibitin complex.
Nat.Cell Biol., 2025

PubMed Abstract: Prohibitins are a highly conserved family of proteins that have been implicated in a variety of functions including mitochondrial stress signalling and housekeeping, cell cycle progression, apoptosis, lifespan regulation and many others. The human prohibitins prohibitin 1 and prohibitin 2 have been proposed to act as scaffolds within the mitochondrial inner membrane, but their molecular organization has remained elusive. Here we determined the molecular organization of the human prohibitin complex within the mitochondrial inner membrane using an integrative structural biology approach combining quantitative western blotting, cryo-electron tomography, subtomogram averaging and molecular modelling. The proposed bell-shaped structure consists of 11 alternating prohibitin 1 and prohibitin 2 molecules. This study reveals an average of about 43 prohibitin complexes per crista, covering 1-3% of the crista membrane area. These findings provide a structural basis for understanding the functional contributions of prohibitins to the integrity and spatial organization of the mitochondrial inner membrane.

PubMed: 40119201
DOI: 10.1038/s41556-025-01620-1

Experimental method

ELECTRON MICROSCOPY (16.3 Å)