8RC0

Structure of the human 20S U5 snRNP

Summary for 8RC0

• Entry DOI: 10.2210/pdb8rc0/pdb
• EMDB information: 19041
• Descriptor: CD2 antigen cytoplasmic tail-binding protein 2, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, ... (16 entities in total)
• Functional Keywords: snrnp, cd2bp2, spliceosome, u5, splicing
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 15
• Total formula weight: 1040565.45

Authors

Schneider, S.,Galej, W.P. (deposition date: 2023-12-05, release date: 2024-03-27, Last modification date: 2024-10-23)

Primary citation

Schneider, S.,Brandina, I.,Peter, D.,Lagad, S.,Fraudeau, A.,Portell-Montserrat, J.,Tholen, J.,Zhao, J.,Galej, W.P.
Structure of the human 20S U5 snRNP.
Nat.Struct.Mol.Biol., 31:752-756, 2024

PubMed Abstract: The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.

PubMed: 38467877
DOI: 10.1038/s41594-024-01250-5

Experimental method

ELECTRON MICROSCOPY (3.2 Å)