8RC0

Structure of the human 20S U5 snRNP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000244	biological_process	spliceosomal tri-snRNP complex assembly
A	0000375	biological_process	RNA splicing, via transesterification reactions
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005682	cellular_component	U5 snRNP
A	0006397	biological_process	mRNA processing
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008380	biological_process	RNA splicing
A	0016020	cellular_component	membrane
A	0016607	cellular_component	nuclear speck
A	0017070	molecular_function	U6 snRNA binding
A	0030532	cellular_component	small nuclear ribonucleoprotein complex
A	0030619	molecular_function	U1 snRNA binding
A	0030620	molecular_function	U2 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
A	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0070530	molecular_function	K63-linked polyubiquitin modification-dependent protein binding
A	0071005	cellular_component	U2-type precatalytic spliceosome
A	0071006	cellular_component	U2-type catalytic step 1 spliceosome
A	0071007	cellular_component	U2-type catalytic step 2 spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0097157	molecular_function	pre-mRNA intronic binding
A	0140492	molecular_function	metal-dependent deubiquitinase activity
B	0000354	biological_process	cis assembly of pre-catalytic spliceosome
B	0000388	biological_process	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0001649	biological_process	osteoblast differentiation
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0003724	molecular_function	RNA helicase activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005681	cellular_component	spliceosomal complex
B	0005682	cellular_component	U5 snRNP
B	0006397	biological_process	mRNA processing
B	0008380	biological_process	RNA splicing
B	0016020	cellular_component	membrane
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042802	molecular_function	identical protein binding
B	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
B	0071005	cellular_component	U2-type precatalytic spliceosome
B	0071006	cellular_component	U2-type catalytic step 1 spliceosome
B	0071013	cellular_component	catalytic step 2 spliceosome
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0003723	molecular_function	RNA binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005681	cellular_component	spliceosomal complex
C	0005829	cellular_component	cytosol
C	0006397	biological_process	mRNA processing
C	0008380	biological_process	RNA splicing
C	0015030	cellular_component	Cajal body
C	0016020	cellular_component	membrane
C	0016607	cellular_component	nuclear speck
C	0030623	molecular_function	U5 snRNA binding
C	0042220	biological_process	response to cocaine
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
C	0071005	cellular_component	U2-type precatalytic spliceosome
C	0071007	cellular_component	U2-type catalytic step 2 spliceosome
C	0071013	cellular_component	catalytic step 2 spliceosome
C	0071466	biological_process	cellular response to xenobiotic stimulus
D	0000354	biological_process	cis assembly of pre-catalytic spliceosome
D	0000375	biological_process	RNA splicing, via transesterification reactions
D	0000398	biological_process	mRNA splicing, via spliceosome
D	0000785	cellular_component	chromatin
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0003724	molecular_function	RNA helicase activity
D	0003729	molecular_function	mRNA binding
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005681	cellular_component	spliceosomal complex
D	0005682	cellular_component	U5 snRNP
D	0005694	cellular_component	chromosome
D	0005730	cellular_component	nucleolus
D	0006397	biological_process	mRNA processing
D	0008380	biological_process	RNA splicing
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
D	0062176	biological_process	R-loop processing
D	0070062	cellular_component	extracellular exosome
D	0071013	cellular_component	catalytic step 2 spliceosome
E	0000244	biological_process	spliceosomal tri-snRNP complex assembly
E	0000245	biological_process	spliceosomal complex assembly
E	0000375	biological_process	RNA splicing, via transesterification reactions
E	0000398	biological_process	mRNA splicing, via spliceosome
E	0003723	molecular_function	RNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005681	cellular_component	spliceosomal complex
E	0005682	cellular_component	U5 snRNP
E	0006396	biological_process	RNA processing
E	0006397	biological_process	mRNA processing
E	0006403	biological_process	RNA localization
E	0008380	biological_process	RNA splicing
E	0016020	cellular_component	membrane
E	0016607	cellular_component	nuclear speck
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0042802	molecular_function	identical protein binding
E	0043021	molecular_function	ribonucleoprotein complex binding
E	0045944	biological_process	positive regulation of transcription by RNA polymerase II
E	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
E	0071005	cellular_component	U2-type precatalytic spliceosome
E	0071013	cellular_component	catalytic step 2 spliceosome
F	0000244	biological_process	spliceosomal tri-snRNP complex assembly
F	0001650	cellular_component	fibrillar center
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005682	cellular_component	U5 snRNP
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006397	biological_process	mRNA processing
F	0008380	biological_process	RNA splicing
F	0016607	cellular_component	nuclear speck
F	0043021	molecular_function	ribonucleoprotein complex binding
F	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
G	0000375	biological_process	RNA splicing, via transesterification reactions
G	0000398	biological_process	mRNA splicing, via spliceosome
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005681	cellular_component	spliceosomal complex
G	0005682	cellular_component	U5 snRNP
G	0005732	cellular_component	sno(s)RNA-containing ribonucleoprotein complex
G	0005829	cellular_component	cytosol
G	0006396	biological_process	RNA processing
G	0006397	biological_process	mRNA processing
G	0008380	biological_process	RNA splicing
G	0016607	cellular_component	nuclear speck
G	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
G	0071007	cellular_component	U2-type catalytic step 2 spliceosome
G	0071013	cellular_component	catalytic step 2 spliceosome
h	0000387	biological_process	spliceosomal snRNP assembly
h	0000398	biological_process	mRNA splicing, via spliceosome
h	0003723	molecular_function	RNA binding
h	0005515	molecular_function	protein binding
h	0005634	cellular_component	nucleus
h	0005654	cellular_component	nucleoplasm
h	0005681	cellular_component	spliceosomal complex
h	0005682	cellular_component	U5 snRNP
h	0005683	cellular_component	U7 snRNP
h	0005684	cellular_component	U2-type spliceosomal complex
h	0005685	cellular_component	U1 snRNP
h	0005686	cellular_component	U2 snRNP
h	0005687	cellular_component	U4 snRNP
h	0005689	cellular_component	U12-type spliceosomal complex
h	0005697	cellular_component	telomerase holoenzyme complex
h	0005737	cellular_component	cytoplasm
h	0005829	cellular_component	cytosol
h	0006397	biological_process	mRNA processing
h	0006479	biological_process	protein methylation
h	0008380	biological_process	RNA splicing
h	0030532	cellular_component	small nuclear ribonucleoprotein complex
h	0034709	cellular_component	methylosome
h	0034719	cellular_component	SMN-Sm protein complex
h	0036261	biological_process	7-methylguanosine cap hypermethylation
h	0043229	cellular_component	intracellular organelle
h	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
h	0070034	molecular_function	telomerase RNA binding
h	0070990	molecular_function	snRNP binding
h	0071004	cellular_component	U2-type prespliceosome
h	0071005	cellular_component	U2-type precatalytic spliceosome
h	0071007	cellular_component	U2-type catalytic step 2 spliceosome
h	0071013	cellular_component	catalytic step 2 spliceosome
h	0071204	cellular_component	histone pre-mRNA 3'end processing complex
h	0071208	molecular_function	histone pre-mRNA DCP binding
h	1903241	biological_process	U2-type prespliceosome assembly
h	1990446	molecular_function	U1 snRNP binding
h	1990447	molecular_function	U2 snRNP binding
i	0000243	cellular_component	commitment complex
i	0000245	biological_process	spliceosomal complex assembly
i	0000387	biological_process	spliceosomal snRNP assembly
i	0000398	biological_process	mRNA splicing, via spliceosome
i	0003723	molecular_function	RNA binding
i	0005515	molecular_function	protein binding
i	0005634	cellular_component	nucleus
i	0005654	cellular_component	nucleoplasm
i	0005681	cellular_component	spliceosomal complex
i	0005682	cellular_component	U5 snRNP
i	0005684	cellular_component	U2-type spliceosomal complex
i	0005685	cellular_component	U1 snRNP
i	0005686	cellular_component	U2 snRNP
i	0005687	cellular_component	U4 snRNP
i	0005689	cellular_component	U12-type spliceosomal complex
i	0005737	cellular_component	cytoplasm
i	0005829	cellular_component	cytosol
i	0006396	biological_process	RNA processing
i	0006397	biological_process	mRNA processing
i	0008380	biological_process	RNA splicing
i	0030532	cellular_component	small nuclear ribonucleoprotein complex
i	0034709	cellular_component	methylosome
i	0034715	cellular_component	pICln-Sm protein complex
i	0034719	cellular_component	SMN-Sm protein complex
i	0036261	biological_process	7-methylguanosine cap hypermethylation
i	0043229	cellular_component	intracellular organelle
i	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
i	0071005	cellular_component	U2-type precatalytic spliceosome
i	0071007	cellular_component	U2-type catalytic step 2 spliceosome
i	0071011	cellular_component	precatalytic spliceosome
i	0071013	cellular_component	catalytic step 2 spliceosome
i	0097526	cellular_component	spliceosomal tri-snRNP complex
i	1903241	biological_process	U2-type prespliceosome assembly
j	0000245	biological_process	spliceosomal complex assembly
j	0000387	biological_process	spliceosomal snRNP assembly
j	0000398	biological_process	mRNA splicing, via spliceosome
j	0003723	molecular_function	RNA binding
j	0005515	molecular_function	protein binding
j	0005634	cellular_component	nucleus
j	0005654	cellular_component	nucleoplasm
j	0005681	cellular_component	spliceosomal complex
j	0005682	cellular_component	U5 snRNP
j	0005684	cellular_component	U2-type spliceosomal complex
j	0005685	cellular_component	U1 snRNP
j	0005686	cellular_component	U2 snRNP
j	0005687	cellular_component	U4 snRNP
j	0005689	cellular_component	U12-type spliceosomal complex
j	0005737	cellular_component	cytoplasm
j	0005829	cellular_component	cytosol
j	0006397	biological_process	mRNA processing
j	0008380	biological_process	RNA splicing
j	0030532	cellular_component	small nuclear ribonucleoprotein complex
j	0034709	cellular_component	methylosome
j	0034715	cellular_component	pICln-Sm protein complex
j	0034719	cellular_component	SMN-Sm protein complex
j	0036261	biological_process	7-methylguanosine cap hypermethylation
j	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
j	0070062	cellular_component	extracellular exosome
j	0071005	cellular_component	U2-type precatalytic spliceosome
j	0071007	cellular_component	U2-type catalytic step 2 spliceosome
j	0071011	cellular_component	precatalytic spliceosome
j	0071013	cellular_component	catalytic step 2 spliceosome
j	1903241	biological_process	U2-type prespliceosome assembly
k	0000243	cellular_component	commitment complex
k	0000387	biological_process	spliceosomal snRNP assembly
k	0000398	biological_process	mRNA splicing, via spliceosome
k	0003723	molecular_function	RNA binding
k	0005515	molecular_function	protein binding
k	0005634	cellular_component	nucleus
k	0005654	cellular_component	nucleoplasm
k	0005681	cellular_component	spliceosomal complex
k	0005682	cellular_component	U5 snRNP
k	0005683	cellular_component	U7 snRNP
k	0005684	cellular_component	U2-type spliceosomal complex
k	0005685	cellular_component	U1 snRNP
k	0005686	cellular_component	U2 snRNP
k	0005687	cellular_component	U4 snRNP
k	0005689	cellular_component	U12-type spliceosomal complex
k	0005697	cellular_component	telomerase holoenzyme complex
k	0005737	cellular_component	cytoplasm
k	0005829	cellular_component	cytosol
k	0006396	biological_process	RNA processing
k	0006397	biological_process	mRNA processing
k	0006479	biological_process	protein methylation
k	0008380	biological_process	RNA splicing
k	0016604	cellular_component	nuclear body
k	0019899	molecular_function	enzyme binding
k	0030532	cellular_component	small nuclear ribonucleoprotein complex
k	0034709	cellular_component	methylosome
k	0034715	cellular_component	pICln-Sm protein complex
k	0034719	cellular_component	SMN-Sm protein complex
k	0036261	biological_process	7-methylguanosine cap hypermethylation
k	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
k	0070034	molecular_function	telomerase RNA binding
k	0071005	cellular_component	U2-type precatalytic spliceosome
k	0071007	cellular_component	U2-type catalytic step 2 spliceosome
k	0071011	cellular_component	precatalytic spliceosome
k	0071013	cellular_component	catalytic step 2 spliceosome
k	0071208	molecular_function	histone pre-mRNA DCP binding
k	0071209	molecular_function	U7 snRNA binding
k	0097526	cellular_component	spliceosomal tri-snRNP complex
k	1903241	biological_process	U2-type prespliceosome assembly
k	1990446	molecular_function	U1 snRNP binding
l	0000245	biological_process	spliceosomal complex assembly
l	0000387	biological_process	spliceosomal snRNP assembly
l	0000398	biological_process	mRNA splicing, via spliceosome
l	0003723	molecular_function	RNA binding
l	0005515	molecular_function	protein binding
l	0005634	cellular_component	nucleus
l	0005654	cellular_component	nucleoplasm
l	0005681	cellular_component	spliceosomal complex
l	0005682	cellular_component	U5 snRNP
l	0005683	cellular_component	U7 snRNP
l	0005684	cellular_component	U2-type spliceosomal complex
l	0005685	cellular_component	U1 snRNP
l	0005686	cellular_component	U2 snRNP
l	0005687	cellular_component	U4 snRNP
l	0005689	cellular_component	U12-type spliceosomal complex
l	0005697	cellular_component	telomerase holoenzyme complex
l	0005737	cellular_component	cytoplasm
l	0005829	cellular_component	cytosol
l	0006397	biological_process	mRNA processing
l	0008380	biological_process	RNA splicing
l	0030532	cellular_component	small nuclear ribonucleoprotein complex
l	0034709	cellular_component	methylosome
l	0034715	cellular_component	pICln-Sm protein complex
l	0034719	cellular_component	SMN-Sm protein complex
l	0036261	biological_process	7-methylguanosine cap hypermethylation
l	0043229	cellular_component	intracellular organelle
l	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
l	0071005	cellular_component	U2-type precatalytic spliceosome
l	0071007	cellular_component	U2-type catalytic step 2 spliceosome
l	0071011	cellular_component	precatalytic spliceosome
l	0071013	cellular_component	catalytic step 2 spliceosome
l	1903241	biological_process	U2-type prespliceosome assembly
m	0000387	biological_process	spliceosomal snRNP assembly
m	0000398	biological_process	mRNA splicing, via spliceosome
m	0003723	molecular_function	RNA binding
m	0005515	molecular_function	protein binding
m	0005634	cellular_component	nucleus
m	0005654	cellular_component	nucleoplasm
m	0005681	cellular_component	spliceosomal complex
m	0005682	cellular_component	U5 snRNP
m	0005683	cellular_component	U7 snRNP
m	0005684	cellular_component	U2-type spliceosomal complex
m	0005685	cellular_component	U1 snRNP
m	0005686	cellular_component	U2 snRNP
m	0005687	cellular_component	U4 snRNP
m	0005689	cellular_component	U12-type spliceosomal complex
m	0005737	cellular_component	cytoplasm
m	0005829	cellular_component	cytosol
m	0006397	biological_process	mRNA processing
m	0008380	biological_process	RNA splicing
m	0030532	cellular_component	small nuclear ribonucleoprotein complex
m	0034709	cellular_component	methylosome
m	0034715	cellular_component	pICln-Sm protein complex
m	0034719	cellular_component	SMN-Sm protein complex
m	0036261	biological_process	7-methylguanosine cap hypermethylation
m	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
m	0071005	cellular_component	U2-type precatalytic spliceosome
m	0071007	cellular_component	U2-type catalytic step 2 spliceosome
m	0071013	cellular_component	catalytic step 2 spliceosome
m	0120114	cellular_component	Sm-like protein family complex
m	1903241	biological_process	U2-type prespliceosome assembly
n	0000245	biological_process	spliceosomal complex assembly
n	0000387	biological_process	spliceosomal snRNP assembly
n	0000398	biological_process	mRNA splicing, via spliceosome
n	0003723	molecular_function	RNA binding
n	0005515	molecular_function	protein binding
n	0005634	cellular_component	nucleus
n	0005654	cellular_component	nucleoplasm
n	0005681	cellular_component	spliceosomal complex
n	0005682	cellular_component	U5 snRNP
n	0005683	cellular_component	U7 snRNP
n	0005684	cellular_component	U2-type spliceosomal complex
n	0005685	cellular_component	U1 snRNP
n	0005686	cellular_component	U2 snRNP
n	0005687	cellular_component	U4 snRNP
n	0005689	cellular_component	U12-type spliceosomal complex
n	0005737	cellular_component	cytoplasm
n	0005829	cellular_component	cytosol
n	0006397	biological_process	mRNA processing
n	0008380	biological_process	RNA splicing
n	0030532	cellular_component	small nuclear ribonucleoprotein complex
n	0034709	cellular_component	methylosome
n	0034719	cellular_component	SMN-Sm protein complex
n	0036261	biological_process	7-methylguanosine cap hypermethylation
n	0043186	cellular_component	P granule
n	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
n	0071004	cellular_component	U2-type prespliceosome
n	0071005	cellular_component	U2-type precatalytic spliceosome
n	0071007	cellular_component	U2-type catalytic step 2 spliceosome
n	0071011	cellular_component	precatalytic spliceosome
n	0071013	cellular_component	catalytic step 2 spliceosome
n	0097526	cellular_component	spliceosomal tri-snRNP complex
n	1903241	biological_process	U2-type prespliceosome assembly

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADRmI

Chain	Residue	Details
D	VAL547-ILE555

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LASAgfDrLILLWNV

Chain	Residue	Details
G	LEU81-VAL95
G	LEU124-SER138
G	VAL167-ILE181
G	ILE208-LEU222
G	ILE300-THR314

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
i	LYS86
B	ALA1350
C	ASN258

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
B	SER17

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER26
G	LYS270

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18318008, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19367720, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
B	SER225
D	SER109
j	LYS8

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	THR389
B	THR1428
D	LYS811

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:F1LNJ2

Chain	Residue	Details
B	TYR709

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
B	LYS971

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q6P4T2

Chain	Residue	Details
B	THR1765

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
B	SER2002

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
B	THR2131

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
B	SER2133
B	SER2135

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
B	LYS46

---