8QQ3

Streptavidin with a Ni-cofactor

Summary for 8QQ3

Entry DOI	10.2210/pdb8qq3/pdb
Descriptor	Streptavidin, 4-[4-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]butylamino]-~{N}1,~{N}1'-di(quinolin-8-yl)cyclohexane-1,1-dicarboxamide, NICKEL (II) ION, ... (4 entities in total)
Functional Keywords	artificial metalloenzymes, metal binding protein
Biological source	Streptomyces avidinii
Total number of polymer chains	4
Total formula weight	69003.38
Authors	Zhang, K.,Jakob, R.P.,Ward, T.R. (deposition date: 2023-10-03, release date: 2024-02-07, Last modification date: 2024-02-21)
Primary citation	Yu, K.,Zhang, K.,Jakob, R.P.,Maier, T.,Ward, T.R. An artificial nickel chlorinase based on the biotin-streptavidin technology. Chem.Commun.(Camb.), 60:1944-1947, 2024 Cited by PubMed Abstract: Herein, we report on an artificial nickel chlorinase (ANCase) resulting from anchoring a biotinylated nickel-based cofactor within streptavidin (Sav). The resulting ANCase was efficient for the chlorination of diverse C(sp)-H bonds. Guided by the X-ray analysis of the ANCase, the activity of the artificial chlorinase could be significantly improved. This approach opens interesting perspectives for late-stage functionalization of organic intermediates as it complements biocatalytic chlorination strategies. PubMed: 38277163 DOI: 10.1039/d3cc05847f PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.6 Å)

Structure validation