8QMA

Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba

これはPDB形式変換不可エントリーです。

8QMA の概要

• エントリーDOI: 10.2210/pdb8qma/pdb
• EMDBエントリー: 18496
• 分子名称: PAP4, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta'', ... (20 entities in total)
• 機能のキーワード: transcription, chloroplasts, gene expression, rna, polymerase
• 由来する生物種: Sinapis alba; 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 1117948.92

構造登録者

do Prado, P.F.V.,Ahrens, F.M.,Pfannschmidt, T.,Hillen, H.S. (登録日: 2023-09-21, 公開日: 2024-03-06, 最終更新日: 2025-07-09)

主引用文献

do Prado, P.F.V.,Ahrens, F.M.,Liebers, M.,Ditz, N.,Braun, H.P.,Pfannschmidt, T.,Hillen, H.S.
Structure of the multi-subunit chloroplast RNA polymerase.
Mol.Cell, 84:910-, 2024

PubMed Abstract: Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.

PubMed: 38428434
DOI: 10.1016/j.molcel.2024.02.003

実験手法

ELECTRON MICROSCOPY (3.5 Å)