8QLD

Bacteriophage T5 dUTPase mutant with loop deletion (30-35 aa)

Summary for 8QLD

• Entry DOI: 10.2210/pdb8qld/pdb
• Related: 8QKY
• Descriptor: Deoxyuridine 5'-triphosphate nucleotidohydrolase, SULFATE ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: bacteriophage, t5, dutpase, deoxyuridine triphosphate nucleotidohydrolases, dutp, hydrolase
• Biological source: Escherichia phage T5
• Total number of polymer chains: 3
• Total formula weight: 48174.66

Authors

Gabdulkhakov, A.G.,Dzhus, U.F.,Selikhanov, G.K.,Glukhov, A.S. (deposition date: 2023-09-19, release date: 2024-01-17, Last modification date: 2024-01-31)

Primary citation

Glukhov, A.,Marchenkov, V.,Dzhus, U.,Krutilina, A.,Selikhanov, G.,Gabdulkhakov, A.
Bacteriophage T5 dUTPase: Combination of Common Enzymatic and Novel Functions.
Int J Mol Sci, 25:-, 2024

PubMed Abstract: The main function of dUTPases is to regulate the cellular levels of dUTP and dTTP, thereby playing a crucial role in DNA repair mechanisms. Despite the fact that mutant organisms with obliterated dUTPase enzymatic activity remain viable, it is not possible to completely knock out the gene due to the lethal consequences of such a mutation for the organism. As a result, it is considered that this class of enzymes performs an additional function that is essential for the organism's survival. In this study, we provide evidence that the dUTPase of bacteriophage T5 fulfills a supplemental function, in addition to its canonical role. We determined the crystal structure of bacteriophage T5 dUTPase with a resolution of 2.0 Å, and we discovered a distinct short loop consisting of six amino acid residues, representing a unique structural feature specific to the T5-like phages dUTPases. The removal of this element did not affect the overall structure of the homotrimer, but it had significant effects on the development of the phage. Furthermore, it was shown that the enzymatic function and the novel function of the bacteriophage T5 dUTPase are unrelated and independent from each other.

PubMed: 38255966
DOI: 10.3390/ijms25020892

Experimental method

X-RAY DIFFRACTION (2.1 Å)