8QLD
Bacteriophage T5 dUTPase mutant with loop deletion (30-35 aa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU PhotonJet-S |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2023-09-12 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54178 |
Spacegroup name | P 65 |
Unit cell lengths | 88.726, 88.726, 99.999 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.001 - 2.100 |
Rwork | 0.221 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.342 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.094 | 0.510 |
Number of reflections | 26147 | 2150 |
<I/σ(I)> | 13.4 | 3.2 |
Completeness [%] | 99.1 | |
Redundancy | 9 | |
CC(1/2) | 0.998 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 0.085 M HEPES sodium, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate, 15% v/v glycerol |