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8QKV

SWR1-nucleosome complex in configuration 2

This is a non-PDB format compatible entry.
Summary for 8QKV
Entry DOI10.2210/pdb8qkv/pdb
EMDB information18472
DescriptorHistone H3, Vacuolar protein sorting-associated protein 71, RuvB-like protein 1, ... (16 entities in total)
Functional Keywordschromatin remodelling complex, nucleosome, protein-dna complex, dna binding protein
Biological sourceSaccharomyces cerevisiae S288C
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Total number of polymer chains20
Total formula weight825471.66
Authors
Jalal, A.S.B.,Wigley, D.B. (deposition date: 2023-09-18, release date: 2024-10-02, Last modification date: 2024-11-20)
Primary citationGirvan, P.,Jalal, A.S.B.,McCormack, E.A.,Skehan, M.T.,Knight, C.L.,Wigley, D.B.,Rueda, D.S.
Nucleosome flipping drives kinetic proofreading and processivity by SWR1.
Nature, 2024
Cited by
PubMed Abstract: The yeast SWR1 complex catalyses the exchange of histone H2A-H2B dimers in nucleosomes, with Htz1-H2B dimers. Here we used single-molecule analysis to demonstrate two-step double exchange of the two H2A-H2B dimers in a canonical yeast nucleosome with Htz1-H2B dimers, and showed that double exchange can be processive without release of the nucleosome from the SWR1 complex. Further analysis showed that bound nucleosomes flip between two states, with each presenting a different face, and hence histone dimer, to SWR1. The bound dwell time is longer when an H2A-H2B dimer is presented for exchange than when presented with an Htz1-H2B dimer. A hexasome intermediate in the reaction is bound to the SWR1 complex in a single orientation with the 'empty' site presented for dimer insertion. Cryo-electron microscopy analysis revealed different populations of complexes showing nucleosomes caught 'flipping' between different conformations without release, each placing a different dimer into position for exchange, with the Swc2 subunit having a key role in this process. Together, the data reveal a processive mechanism for double dimer exchange that explains how SWR1 can 'proofread' the dimer identities within nucleosomes.
PubMed: 39506114
DOI: 10.1038/s41586-024-08152-y
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (4.7 Å)
Structure validation

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PDB entries from 2024-11-20

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