8Q8O

PA14_16140 protein: the regulator of an operon involved in the biofilm formation in PA14 P. aeruginosa

Summary for 8Q8O

• Entry DOI: 10.2210/pdb8q8o/pdb
• Descriptor: DUF2170 family protein, GLYCEROL, Tb-Xo4, ... (4 entities in total)
• Functional Keywords: regulator, biofilm, pseudomonas, chaperone
• Biological source: Pseudomonas aeruginosa UCBPP-PA14
• Total number of polymer chains: 2
• Total formula weight: 32321.99

Authors

Broutin, I.,Phan, G.,Girard, E. (deposition date: 2023-08-18, release date: 2024-02-28, Last modification date: 2024-04-03)

Primary citation

Wallart, L.,Ben Mlouka, M.A.,Saffiedine, B.,Coquet, L.,Le, H.,Hardouin, J.,Jouenne, T.,Phan, G.,Kiefer-Meyer, M.C.,Girard, E.,Broutin, I.,Cosette, P.
BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa .
Front Microbiol, 15:1332448-1332448, 2024

PubMed Abstract: Previously, we pointed out in PAO1 biofilm cells the accumulation of a hypothetical protein named PA3731 and showed that the deletion of the corresponding gene impacted its biofilm formation capacity. PA3731 belongs to a cluster of 4 genes ( to ) that we named for "Biofilm Associated Cluster." The present study focuses on the PA14_16140 protein, i.e., the PA3732 (BacA) homolog in the PA14 strain. The role of BacA in rhamnolipid secretion, biofilm formation and virulence, was confirmed by phenotypic experiments with a mutant. Additional investigations allow to advance that the system involves in fact 6 genes organized in operon, i.e., to At a molecular level, quantitative proteomic studies revealed an accumulation of the BAC cognate partners by the sessile mutant, suggesting a negative control of BacA toward the operon. Finally, a first crystallographic structure of BacA was obtained revealing a structure homologous to chaperones or/and regulatory proteins.

PubMed: 38505547
DOI: 10.3389/fmicb.2024.1332448

Experimental method

X-RAY DIFFRACTION (2.7 Å)