8Q6K
Human IgD Fab in complex with an orthosteric inhibitor of Phl p 7
Summary for 8Q6K
| Entry DOI | 10.2210/pdb8q6k/pdb |
| Descriptor | Human IgD Fab light chain, Human IgD Fab heavy chain, Nanobody 072, ... (7 entities in total) |
| Functional Keywords | fab, phl p 7, nanobody, igd, immune system |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 3 |
| Total formula weight | 64943.72 |
| Authors | Davies, A.M.,Vester, S.K.,McDonnell, J.M. (deposition date: 2023-08-11, release date: 2023-12-06, Last modification date: 2024-10-16) |
| Primary citation | Vester, S.K.,Davies, A.M.,Beavil, R.L.,Sandhar, B.S.,Beavil, A.J.,Gould, H.J.,Sutton, B.J.,McDonnell, J.M. Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor. Antibodies, 12:-, 2023 Cited by PubMed Abstract: We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described. PubMed: 37987253DOI: 10.3390/antib12040075 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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