8PKP

Cryo-EM structure of the apo Anaphase-promoting complex/cyclosome (APC/C) at 3.2 Angstrom resolution

8PKP の概要

• エントリーDOI: 10.2210/pdb8pkp/pdb
• EMDBエントリー: 13931 17751 3388
• 分子名称: Anaphase-promoting complex subunit 1, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 7, ... (15 entities in total)
• 機能のキーワード: apc/c, cyclosome, cdc20, cdh1, ubiquitination, emi1, mitosis, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 1166029.12

構造登録者

Hoefler, A.,Yu, J.,Chang, L.,Zhang, Z.,Yang, J.,Boland, A.,Barford, D. (登録日: 2023-06-27, 公開日: 2023-07-19, 最終更新日: 2025-01-29)

主引用文献

Hofler, A.,Yu, J.,Yang, J.,Zhang, Z.,Chang, L.,McLaughlin, S.H.,Grime, G.W.,Garman, E.F.,Boland, A.,Barford, D.
Cryo-EM structures of apo-APC/C and APC/C CDH1:EMI1 complexes provide insights into APC/C regulation.
Nat Commun, 15:10074-10074, 2024

PubMed Abstract: APC/C is a multi-subunit complex that functions as a master regulator of cell division. It controls progression through the cell cycle by timely marking mitotic cyclins and other cell cycle regulatory proteins for degradation. The APC/C itself is regulated by the sequential action of its coactivator subunits CDC20 and CDH1, post-translational modifications, and its inhibitory binding partners EMI1 and the mitotic checkpoint complex. In this study, we took advantage of developments in cryo-electron microscopy to determine the structures of human APC/C and apo-APC/C at 2.9 Å and 3.2 Å resolution, respectively, providing insights into the regulation of APC/C activity. The high-resolution maps allow the unambiguous assignment of an α-helix to the N-terminus of CDH1 (CDH1) in the APC/C ternary complex. We also identify a zinc-binding module in APC2 that confers structural stability to the complex, and we confirm the presence of zinc ions experimentally. Finally, due to the higher resolution and well defined density of these maps, we are able to build, aided by AlphaFold predictions, several intrinsically disordered regions in different APC/C subunits that likely play a role in proper APC/C assembly and regulation of its activity.

PubMed: 39567505
DOI: 10.1038/s41467-024-54398-5

実験手法

ELECTRON MICROSCOPY (3.2 Å)