8PK6
INTS13 complex with ZNF655
Summary for 8PK6
| Entry DOI | 10.2210/pdb8pk6/pdb |
| Descriptor | Integrator complex subunit 13, Zinc finger protein 655 (2 entities in total) |
| Functional Keywords | protein complex, dna binding protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 6 |
| Total formula weight | 93647.47 |
| Authors | Sabath, K.,Jonas, S. (deposition date: 2023-06-25, release date: 2024-07-03, Last modification date: 2024-11-13) |
| Primary citation | Sabath, K.,Nabih, A.,Arnold, C.,Moussa, R.,Domjan, D.,Zaugg, J.B.,Jonas, S. Basis of gene-specific transcription regulation by the Integrator complex. Mol.Cell, 84:2525-, 2024 Cited by PubMed Abstract: The Integrator complex attenuates gene expression via the premature termination of RNA polymerase II (RNAP2) at promoter-proximal pausing sites. It is required for stimulus response, cell differentiation, and neurodevelopment, but how gene-specific and adaptive regulation by Integrator is achieved remains unclear. Here, we identify two sites on human Integrator subunits 13/14 that serve as binding hubs for sequence-specific transcription factors (TFs) and other transcription effector complexes. When Integrator is attached to paused RNAP2, these hubs are positioned upstream of the transcription bubble, consistent with simultaneous TF-promoter tethering. The TFs co-localize with Integrator genome-wide, increase Integrator abundance on target genes, and co-regulate responsive transcriptional programs. For instance, sensory cilia formation induced by glucose starvation depends on Integrator-TF contacts. Our data suggest TF-mediated promoter recruitment of Integrator as a widespread mechanism for targeted transcription regulation. PubMed: 38906142DOI: 10.1016/j.molcel.2024.05.027 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.21 Å) |
Structure validation
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