8PK6

INTS13 complex with ZNF655

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	126.997, 188.103, 52.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.720 - 3.210
R-factor	0.3007
Rwork	0.299
R-free	0.33290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	3.330
High resolution limit [Å]	3.210	3.220
Number of reflections	21358	2085
<I/σ(I)>	9.75
Completeness [%]	99.5
Redundancy	13.4
CC(1/2)	0.999	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM MES pH 6.0, 100 mM MgCl2 and 8% (w/v) polyethylene glycol 6,000