8PDZ
Recombinant Ena3A L-Type endospore appendages
Summary for 8PDZ
| Entry DOI | 10.2210/pdb8pdz/pdb |
| EMDB information | 17627 |
| Descriptor | DUF3992 domain-containing protein (1 entity in total) |
| Functional Keywords | endospore, pili, protein, fiber, spore, appendage, bacillus paranthracis, protein fibril, recombinant |
| Biological source | Bacillus paranthracis |
| Total number of polymer chains | 28 |
| Total formula weight | 324440.20 |
| Authors | Sleutel, M.,Remaut, H. (deposition date: 2023-06-13, release date: 2023-11-08, Last modification date: 2025-09-10) |
| Primary citation | Sleutel, M.,Zegeye, E.D.,Llarena, A.K.,Pradhan, B.,Fislage, M.,O'Sullivan, K.,Van Gerven, N.,Aspholm, M.,Remaut, H. Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain. Nat Commun, 15:7514-7514, 2024 Cited by PubMed Abstract: In pathogenic Bacillota, spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease. A poorly understood aspect of spore-mediated infection is the fibrous structures or 'endospore appendages' (ENAs) that have been seen to decorate the spores of pathogenic Bacilli and Clostridia. Current methodological approaches are opening a window on these long enigmatic structures. Using cryoID, Alphafold modelling and genetic approaches we identify a sub-class of robust ENAs in a Bacillus paranthracis foodborne outbreak strain. We demonstrate that L-ENA are encoded by a rare three-gene cluster (ena3) that contains all components for the self-assembly of ladder-like protein nanofibers of stacked heptameric rings, their anchoring to the exosporium, and their termination in a trimeric 'ruffle' made of a complement C1Q-like BclA paralogue. The role of ENA fibers in spore-spore interaction and the distribution of L-ENA operon as mobile genetic elements in B. cereus s.l. strains suggest that L-ENA fibers may increase the survival, spread and virulence of these strains. PubMed: 39209852DOI: 10.1038/s41467-024-51804-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.32 Å) |
Structure validation
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