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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Recombinant Ena3A L-Type endospore appendages | |||||||||
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![]() | Endospore / Pili / Protein / Fiber / Spore / Appendage / Bacillus paranthracis / PROTEIN FIBRIL / recombinant | |||||||||
Function / homology | Endospore appendages core / Endospore appendages / DUF3992 domain-containing protein![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.32 Å | |||||||||
![]() | Sleutel M / Remaut H | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A novel class of ultra-stable endospore appendages decorated with collagen-like tip fibrillae Authors: Sleutel M / Zegeye ED / Llarena AK / Pradhan B / Fislage M / O'Sullivan K / Aspholm M / Remaut H | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 50.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14 KB 14 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.9 KB | Display | ![]() |
Images | ![]() | 71.8 KB | ||
Masks | ![]() | 103 MB | ![]() | |
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 95.6 MB 95.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 952.5 KB | Display | ![]() |
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Full document | ![]() | 952.1 KB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 23.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8pdzMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.764 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
File | emd_17627_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Density Histograms |
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Sample components
-Entire : Recombinant Ena3A L-type endospore appendage
Entire | Name: Recombinant Ena3A L-type endospore appendage |
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Components |
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-Supramolecule #1: Recombinant Ena3A L-type endospore appendage
Supramolecule | Name: Recombinant Ena3A L-type endospore appendage / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 18.03 kDa/nm |
-Macromolecule #1: DUF3992 domain-containing protein
Macromolecule | Name: DUF3992 domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 28 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 11.58715 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MAQIGNCCTE QLCCVNDAVC CTIILDDTGG TALPIWDDAT TFVINGTIMV ENNGTVGVGP TAALTVNGTA VGGFVVAPGE CRSITMNDI NSIAIVGAGT GTSSVKISFS INYKF UniProtKB: DUF3992 domain-containing protein |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7 / Component - Formula: miliQ |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | JEOL CRYO ARM 300 |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 8528 / Average electron dose: 62.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 60000 |