8PB9

Cryo-EM structure of the c-di-GMP-bound FleQ-FleN master regulator complex from Pseudomonas aeruginosa

8PB9 の概要

• エントリーDOI: 10.2210/pdb8pb9/pdb
• 関連するPDBエントリー: 8p53
• EMDBエントリー: 17445 17581
• 分子名称: Transcriptional regulator FleQ, Antiactivator FleN, 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one), ... (4 entities in total)
• 機能のキーワード: biofilm formation, c-di-gmp signaling, second messengers, bacterial secretion, gene regulation, bebps
• 由来する生物種: Pseudomonas aeruginosa PAO1; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 197698.47

構造登録者

Torres-Sanchez, L.T.,Krasteva, P.V. (登録日: 2023-06-08, 公開日: 2023-12-13, 最終更新日: 2025-10-01)

主引用文献

Torres-Sanchez, L.,Gery Sana, T.,Decossas, M.,Hashem, Y.,Krasteva, P.V.
Structures of the P. aeruginosa FleQ-FleN master regulators reveal large-scale conformational switching in motility and biofilm control.
Proc.Natl.Acad.Sci.USA, 120:e2312276120-e2312276120, 2023

PubMed Abstract: can cause a wide array of chronic and acute infections associated with its ability to rapidly switch between planktonic, biofilm, and dispersed lifestyles, each with a specific arsenal for bacterial survival and virulence. At the cellular level, many of the physiological transitions are orchestrated by the intracellular second messenger c-di-GMP and its receptor-effector FleQ. A bacterial enhancer binding protein, FleQ acts as a master regulator of both flagellar motility and adherence factor secretion and uses remarkably different transcription activation mechanisms depending on its dinucleotide loading state, adenosine triphosphatase (ATPase) activity, interactions with polymerase sigma (σ) factors, and complexation with a second ATPase, FleN. How the FleQ-FleN tandem can exert diverse effects through recognition of a conserved FleQ binding consensus has remained enigmatic. Here, we provide cryogenic electron microscopy (cryo-EM) structures of both c-di-GMP-bound and c-di-GMP-free FleQ-FleN complexes which deepen our understanding of the proteins' (di)nucleotide-dependent conformational switching and fine-tuned roles in gene expression regulation.

PubMed: 38051770
DOI: 10.1073/pnas.2312276120

実験手法

ELECTRON MICROSCOPY (3.3 Å)