8OW1
Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
This is a non-PDB format compatible entry.
Summary for 8OW1
| Entry DOI | 10.2210/pdb8ow1/pdb |
| EMDB information | 17227 17368 17371 17372 17374 17376 |
| Descriptor | Centromere-binding protein 1, Inner kinetochore subunit CTF19, Inner kinetochore subunit OKP1, ... (23 entities in total) |
| Functional Keywords | kinetochore, point centromere, cenp-a nucleosome, topological entrapment, centromeric dna, cell cycle |
| Biological source | Saccharomyces cerevisiae (baker's yeast) More |
| Total number of polymer chains | 42 |
| Total formula weight | 1490633.36 |
| Authors | Dendooven, T.D.,Zhang, Z.,Yang, J.,McLaughlin, S.,Schwabb, J.,Scheres, S.,Yatskevich, S.,Barford, D. (deposition date: 2023-04-26, release date: 2023-08-09, Last modification date: 2025-07-02) |
| Primary citation | Dendooven, T.,Zhang, Z.,Yang, J.,McLaughlin, S.H.,Schwab, J.,Scheres, S.H.W.,Yatskevich, S.,Barford, D. Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere. Sci Adv, 9:eadg7480-eadg7480, 2023 Cited by PubMed Abstract: The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A). This revealed a central CENP-A with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (CDEI) motif by CBF1. The two CCAN protomers are linked through CBF3 forming an arch-like configuration. With a structural mechanism for how CENP-A can also be linked to KMN involving only CENP-QU, we present a model for inner kinetochore assembly onto a point centromere and how it organizes the outer kinetochore for chromosome attachment to the mitotic spindle. PubMed: 37506202DOI: 10.1126/sciadv.adg7480 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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