8ODO

Structure of human guanylylated RTCB in complex with Archease

8ODO の概要

• エントリーDOI: 10.2210/pdb8odo/pdb
• 関連するPDBエントリー: 8BTT 8BTX 8ODP
• 分子名称: RNA-splicing ligase RtcB homolog, Protein archease, GUANOSINE-5'-MONOPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: archease, rtcb, human, ligase, trna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 315912.18

構造登録者

Kopp, J.,Gerber, J.L.,Peschek, J. (登録日: 2023-03-09, 公開日: 2024-03-27, 最終更新日: 2024-11-06)

主引用文献

Gerber, J.L.,Morales Guzman, S.I.,Worf, L.,Hubbe, P.,Kopp, J.,Peschek, J.
Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease.
Nat Commun, 15:2378-2378, 2024

PubMed Abstract: RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex. RNA ligation by the tRNA ligase complex requires GTP-dependent activation of RTCB. This active site guanylylation reaction relies on the activation factor Archease. The mechanistic interplay between both proteins has remained unknown. Here, we report a biochemical and structural analysis of the human RTCB-Archease complex in the pre- and post-activation state. Archease reaches into the active site of RTCB and promotes the formation of a covalent RTCB-GMP intermediate through coordination of GTP and metal ions. During the activation reaction, Archease prevents futile RNA substrate binding to RTCB. Moreover, monomer structures of Archease and RTCB reveal additional states within the RNA ligation mechanism. Taken together, we present structural snapshots along the reaction cycle of the human tRNA ligase.

PubMed: 38493148
DOI: 10.1038/s41467-024-46568-2

実験手法

X-RAY DIFFRACTION (2.2 Å)