8JYD
A genetically encoded sensor based on a bacterial DNA ligase
Summary for 8JYD
| Entry DOI | 10.2210/pdb8jyd/pdb |
| Descriptor | DNA ligase, BETA-NICOTINAMIDE RIBOSE MONOPHOSPHATE (3 entities in total) |
| Functional Keywords | dna ligase, complex, mutant, ligase |
| Biological source | Enterococcus faecalis (strain ATCC 700802 / V583) |
| Total number of polymer chains | 1 |
| Total formula weight | 35950.32 |
| Authors | Chen, L. (deposition date: 2023-07-03, release date: 2024-07-10, Last modification date: 2025-01-29) |
| Primary citation | Chen, L.,Wang, P.,Huang, G.,Cheng, W.,Liu, K.,Yu, Q. Quantitative dynamics of intracellular NMN by genetically encoded biosensor. Biosens.Bioelectron., 267:116842-116842, 2025 Cited by PubMed Abstract: Nicotinamide mononucleotide (NMN) is the direct precursor and a major booster of NAD with increasing applications in NAD- and aging-related pathologies. However, measuring live cell NMN dynamics was not possible, leaving key questions in NMN uptake and intracellular regulation unanswered. Here we developed genetically encoded bioluminescent and fluorescent sensors to quantify subcellular NMN in live cells by engineering specific NMN-responsive protein scaffolds fused to luciferase and fluorescent proteins. The sensor dissected the multimechanistic uptake of exogenous NMN and nicotinamide riboside (NR) in live cells and further measured the NMN levels across different subcellular compartments, as well as the perturbed NMN/NAD ratios by external supplements. Moreover, we measured the NMN regulation by NAD(H) hydrolase Nudts and peroxisomal carrier Pxmp2 and identified Slc25a45 as a potential mitochondrial NMN regulator for its unique fingerprint on the local NMN/NAD ratio. Collectively, the genetically encoded sensors provide a useful tool for visualizing NMN metabolism. PubMed: 39418868DOI: 10.1016/j.bios.2024.116842 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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