8JYD
A genetically encoded sensor based on a bacterial DNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2023-06-26 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.480, 92.480, 74.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.290 - 3.000 |
| R-factor | 0.21706 |
| Rwork | 0.215 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.184 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.290 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 1.632 |
| Rmeas | 0.136 | |
| Rpim | 0.031 | 0.369 |
| Number of reflections | 7642 | 574 |
| <I/σ(I)> | 25.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.2 | |
| CC(1/2) | 0.999 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.0; 20% PEG mono 2000 |
