8JTJ

Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex

Summary for 8JTJ

Entry DOI	10.2210/pdb8jtj/pdb
EMDB information	36646
Descriptor	CRISPR-associated endonuclease Cas9, RNA (139-MER), DNA (29-MER), ... (4 entities in total)
Functional Keywords	rna-binding, dna-binding, complex, dna binding protein-rna-dna complex, dna binding protein/rna/dna
Biological source	Geobacillus stearothermophilus More
Total number of polymer chains	4
Total formula weight	184438.89
Authors	Shen, P.P.,Liu, B.B.,Li, X.,Zhang, L.L.,Chen, C.-C.,Guo, R.-T. (deposition date: 2023-06-21, release date: 2024-06-05, Last modification date: 2025-07-23)
Primary citation	Shen, P.,Zhang, L.,Liu, B.,Li, X.,Wang, C.,Min, J.,Huang, J.W.,Chen, C.C.,Guo, R.T. Structure of Geobacillus stearothermophilus Cas9: Insights into the Catalytic Process and Thermostability of CRISPR-Cas9 Acs Catalysis, 14:13227-13235, 2024 Cited by DOI: 10.1021/acscatal.4c03278 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.08 Å)

Structure validation