8JTJ
Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1095 | 128171.0 | 1 | UniProt (A0A150MP45) | Geobacillus stearothermophilus | |
| 2 | B (B) | RNA (139-MER) | polymer | 139 | 44697.4 | 1 | Geobacillus stearothermophilus | ||
| 3 | C (C) | DNA (29-MER) | polymer | 29 | 8764.6 | 1 | Geobacillus stearothermophilus | ||
| 4 | D (D) | DNA (5'-D(P*GP*GP*GP*CP*GP*CP*GP*AP*A)-3') | polymer | 9 | 2805.8 | 1 | Geobacillus stearothermophilus |
Sequence modifications
A: 1 - 1087 (UniProt: A0A150MP45)
| PDB | External Database | Details |
|---|---|---|
| Ala 219 | Thr 219 | conflict |
| Ala 241 | Thr 241 | conflict |
| Glu 353 | Gly 353 | conflict |
| Ala 582 | His 582 | conflict |
| Leu 1088 | - | expression tag |
| Glu 1089 | - | expression tag |
| His 1090 | - | expression tag |
| His 1091 | - | expression tag |
| His 1092 | - | expression tag |
| His 1093 | - | expression tag |
| His 1094 | - | expression tag |
| His 1095 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 184438.9 | |
| All* | Total formula weight | 184438.9 |
