8JO4

Cryo-EM structure of a Legionella effector complexed with actin and ATP

8JO4 の概要

• エントリーDOI: 10.2210/pdb8jo4/pdb
• EMDBエントリー: 36455
• 分子名称: Substrate of the Dot/Icm secretion system, Actin, alpha skeletal muscle, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: ampylation, toxin
• 由来する生物種: Legionella sainthelensi; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93527.90

構造登録者

Zhou, X.T.,Wang, X.F.,Tan, J.X.,Zhu, Y.Q. (登録日: 2023-06-07, 公開日: 2024-05-01, 最終更新日: 2024-07-24)

主引用文献

Wang, T.,Song, X.,Tan, J.,Xian, W.,Zhou, X.,Yu, M.,Wang, X.,Xu, Y.,Wu, T.,Yuan, K.,Ran, Y.,Yang, B.,Fan, G.,Liu, X.,Zhou, Y.,Zhu, Y.
Legionella effector LnaB is a phosphoryl AMPylase that impairs phosphosignalling.
Nature, 631:393-401, 2024

PubMed Abstract: AMPylation is a post-translational modification in which AMP is added to the amino acid side chains of proteins. Here we show that, with ATP as the ligand and actin as the host activator, the effector protein LnaB of Legionella pneumophila exhibits AMPylase activity towards the phosphoryl group of phosphoribose on PR-Ub that is generated by the SidE family of effectors, and deubiquitinases DupA and DupB in an E1- and E2-independent ubiquitination process. The product of LnaB is further hydrolysed by an ADP-ribosylhydrolase, MavL, to Ub, thereby preventing the accumulation of PR-Ub and ADPR-Ub and protecting canonical ubiquitination in host cells. LnaB represents a large family of AMPylases that adopt a common structural fold, distinct from those of the previously known AMPylases, and LnaB homologues are found in more than 20 species of bacterial pathogens. Moreover, LnaB also exhibits robust phosphoryl AMPylase activity towards phosphorylated residues and produces unique ADPylation modifications in proteins. During infection, LnaB AMPylates the conserved phosphorylated tyrosine residues in the activation loop of the Src family of kinases, which dampens downstream phosphorylation signalling in the host. Structural studies reveal the actin-dependent activation and catalytic mechanisms of the LnaB family of AMPylases. This study identifies, to our knowledge, an unprecedented molecular regulation mechanism in bacterial pathogenesis and protein phosphorylation.

PubMed: 38776962
DOI: 10.1038/s41586-024-07573-z

実験手法

ELECTRON MICROSCOPY (3.04 Å)