8J3R

Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA

Summary for 8J3R

Entry DOI	10.2210/pdb8j3r/pdb
EMDB information	35965
Descriptor	Transposase IS605 OrfB C-terminal domain-containing protein, DNA (37-MER), DNA (38-MER), ... (6 entities in total)
Functional Keywords	crispr-cas, rna binding protein-dna complex, rna binding protein-dna-rna complex, rna binding protein/dna/rna
Biological source	Sulfoacidibacillus thermotolerans More
Total number of polymer chains	5
Total formula weight	161469.33
Authors	Hino, T.,Omura, N.S.,Nakagawa, R.,Togashi, T.,Takeda, N.S.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, H.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O. (deposition date: 2023-04-18, release date: 2023-09-27, Last modification date: 2024-10-09)
Primary citation	Hino, T.,Omura, S.N.,Nakagawa, R.,Togashi, T.,Takeda, S.N.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, S.,Kagieva, M.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Karvelis, T.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O. An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis. Cell, 186:4920-4935.e23, 2023 Cited by PubMed: 37776859 DOI: 10.1016/j.cell.2023.08.031 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.95 Å)

Structure validation