8J3R
Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.95 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
