8J3R

Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transposase IS605 OrfB C-terminal domain-containing protein	polymer	432	49978.1	2	UniProt (A0A2U3D0N8) Pfam (PF07282) In PDB	Sulfoacidibacillus thermotolerans
2	D	DNA (37-MER)	polymer	37	11388.4	1		Sulfoacidibacillus thermotolerans
3	E	DNA (38-MER)	polymer	38	11698.5	1		Sulfoacidibacillus thermotolerans
4	C	RNA (118-MER)	polymer	118	38287.8	1		Sulfoacidibacillus thermotolerans
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	A, C	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

A, B: 1 - 422 (UniProt: A0A2U3D0N8)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 123	Ile 123	engineered mutation
Lys 195	Asp 195	engineered mutation
Arg 208	Asp 208	engineered mutation
Ala 232	Val 232	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	161331.0
Non-Polymers*	Number of molecules	4
	Total formula weight	138.3
All*	Total formula weight	161469.3

*Water molecules are not included.