8IYJ
Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm
This is a non-PDB format compatible entry.
Summary for 8IYJ
| Entry DOI | 10.2210/pdb8iyj/pdb |
| EMDB information | 35810 35823 36458 36498 36499 36500 36501 36502 36503 36504 36509 36510 36511 36513 36514 36515 36516 36517 36518 36519 36520 36521 36523 36524 36525 36526 36527 36528 36529 36530 36531 36532 36533 36580 36581 36582 36583 36584 36585 36586 36587 36588 36591 |
| Descriptor | Cilia- and flagella-associated protein 95, Meiosis-specific nuclear structural protein 1, Tektin-2, ... (52 entities in total) |
| Functional Keywords | doublet microtubule, structural protein |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 576 |
| Total formula weight | 27904742.10 |
| Authors | Zhou, L.N.,Gui, M.,Wu, J.P. (deposition date: 2023-04-05, release date: 2023-07-05, Last modification date: 2024-07-03) |
| Primary citation | Zhou, L.,Liu, H.,Liu, S.,Yang, X.,Dong, Y.,Pan, Y.,Xiao, Z.,Zheng, B.,Sun, Y.,Huang, P.,Zhang, X.,Hu, J.,Sun, R.,Feng, S.,Zhu, Y.,Liu, M.,Gui, M.,Wu, J. Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Cell, 186:2897-2910.e19, 2023 Cited by PubMed Abstract: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility. PubMed: 37295417DOI: 10.1016/j.cell.2023.05.009 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
Download full validation report
