8IYJ の概要
| エントリーDOI | 10.2210/pdb8iyj/pdb |
| EMDBエントリー | 35810 35823 36458 36498 36499 36500 36501 36502 36503 36504 36509 36510 36511 36513 36514 36515 36516 36517 36518 36519 36520 36521 36523 36524 36525 36526 36527 36528 36529 36530 36531 36532 36533 36580 36581 36582 36583 36584 36585 36586 36587 36588 36591 |
| 分子名称 | Cilia- and flagella-associated protein 95, Meiosis-specific nuclear structural protein 1, Tektin-2, ... (52 entities in total) |
| 機能のキーワード | doublet microtubule, structural protein |
| 由来する生物種 | Mus musculus (house mouse) 詳細 |
| タンパク質・核酸の鎖数 | 576 |
| 化学式量合計 | 27904742.10 |
| 構造登録者 | |
| 主引用文献 | Zhou, L.,Liu, H.,Liu, S.,Yang, X.,Dong, Y.,Pan, Y.,Xiao, Z.,Zheng, B.,Sun, Y.,Huang, P.,Zhang, X.,Hu, J.,Sun, R.,Feng, S.,Zhu, Y.,Liu, M.,Gui, M.,Wu, J. Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Cell, 186:2897-2910.e19, 2023 Cited by PubMed Abstract: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility. PubMed: 37295417DOI: 10.1016/j.cell.2023.05.009 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.5 Å) |
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