8IT0

Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-2)

Summary for 8IT0

Entry DOI	10.2210/pdb8it0/pdb
EMDB information	35702
Descriptor	Piwi domain-containing protein, TIR domain-containing protein, DNA (45-mer), ... (4 entities in total)
Functional Keywords	ago, dna/rna, dimer, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna
Biological source	Thermoflavifilum thermophilum More
Total number of polymer chains	8
Total formula weight	264190.99
Authors	Gao, X.,Shang, K.,Zhu, K.,Wang, L.,Mu, Z.,Fu, X.,Yu, X.,Qin, B.,Zhu, H.,Ding, W.,Cui, S. (deposition date: 2023-03-21, release date: 2023-10-18, Last modification date: 2024-02-07)
Primary citation	Gao, X.,Shang, K.,Zhu, K.,Wang, L.,Mu, Z.,Fu, X.,Yu, X.,Qin, B.,Zhu, H.,Ding, W.,Cui, S. Nucleic-acid-triggered NADase activation of a short prokaryotic Argonaute. Nature, 625:822-831, 2024 Cited by PubMed: 37783228 DOI: 10.1038/s41586-023-06665-6 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.5 Å)

Structure validation