8IT0

Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, E)	Piwi domain-containing protein	polymer	507	58304.8	2	UniProt (A0A1I7NFD7)	Thermoflavifilum thermophilum	CrtAgo
2	B, D (B, F)	TIR domain-containing protein	polymer	450	53256.7	2	UniProt (A0A1I7NFG5) Pfam (PF13676)	Thermoflavifilum thermophilum	CrtTIR-APAZ
3	E, G (D, H)	DNA (45-mer)	polymer	45	13721.9	2		synthetic construct
4	F, H (C, G)	RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*U)-3')	polymer	21	6812.0	2		synthetic construct

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	264191.0
All*	Total formula weight	264191.0

*Water molecules are not included.