8IOI

Crystal Structure of PadR- family transcriptional regulator Rv1176c from Mycobacterium tuberculosis H37Rv.

Summary for 8IOI

• Entry DOI: 10.2210/pdb8ioi/pdb
• Descriptor: PadR family transcriptional regulator, GLYCEROL, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: transcriptional regulator, dna binding protein
• Biological source: Mycobacterium tuberculosis H37Rv
• Total number of polymer chains: 2
• Total formula weight: 43338.21

Authors

Yadav, V.,Zohib, M.,Pal, R.K.,Biswal, B.K.,Arora, A. (deposition date: 2023-03-11, release date: 2024-03-13)

Primary citation

Yadav, V.,Zohib, M.,Singh, S.,Pal, R.K.,Tripathi, S.,Jain, A.,Biswal, B.K.,Dasgupta, A.,Arora, A.
Structural and biophysical characterization of PadR family protein Rv1176c of Mycobacterium tuberculosis H37Rv.
Int.J.Biol.Macromol., 263:130455-130455, 2024

PubMed Abstract: Rv1176c of Mycobacterium tuberculosis H37Rv belongs to the PadR-s1 subfamily of the PadR family of protein. Rv1176c forms a stable dimer in solution. Its stability is characterized by a thermal melting transition temperature (Tm) of 39.4 °C. The crystal structure of Rv1176c was determined at a resolution of 2.94 Å, with two monomers in the asymmetric unit. Each monomer has a characteristic N-terminal winged-helix-turn-helix DNA-binding domain. Rv1176c C-terminal is a coiled-coil dimerization domain formed of α-helices α5 to α7. In the Rv1176c dimer, there is domain-swapping of the C-terminal domain in comparison to other PadR homologs. In the dimer, there is a long inter-subunit tunnel in which different ligands can bind. Rv1176c was found to bind to the promoter region of its own gene with high specificity. M. smegmatis MC 155 genome lacks homolog of Rv1176c. Therefore, it was used as a surrogate to characterize the functional role of Rv1176c. Expression of Rv1176c in M. smegmatis MC 155 cells imparted enhanced tolerance towards oxidative stress. Rv1176c expressing M. smegmatis MC 155 cells exhibited enhanced intracellular survival in J774A.1 murine macrophage cells. Overall, our studies demonstrate Rv1176c to be a PadR-s1 subfamily transcription factor that can moderate the effect of oxidative stress.

PubMed: 38417748
DOI: 10.1016/j.ijbiomac.2024.130455

Experimental method

X-RAY DIFFRACTION (2.94 Å)