8IOI
Crystal Structure of PadR- family transcriptional regulator Rv1176c from Mycobacterium tuberculosis H37Rv.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 45.446, 119.026, 139.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.160 - 2.940 |
R-factor | 0.22196 |
Rwork | 0.217 |
R-free | 0.27023 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.050 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 8284 | 808 |
<I/σ(I)> | 13.19 | |
Completeness [%] | 98.5 | |
Redundancy | 8.2 | |
CC(1/2) | 0.959 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25% PEG 400, 0.1M Tris-HCl pH 8.5, 0.1M Sodium citrate tribasic dihydrate |