8HNI

hnRNP A2/B1 RRMs in complex with telomeric DNA

Summary for 8HNI

• Entry DOI: 10.2210/pdb8hni/pdb
• Descriptor: Heterogeneous nuclear ribonucleoproteins A2/B1, DNA (5'-D(P*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*T)-3') (2 entities in total)
• Functional Keywords: complex, homodimerization, dna g4 quadruplex, dna binding protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 24
• Total formula weight: 291691.61

Authors

Liu, Y.,Abula, A.,Xiao, H.,Guo, H.,Li, T.,Zheng, L.,Chen, B.,Nguyen, H.,Ji, X. (deposition date: 2022-12-07, release date: 2023-11-29)

Primary citation

Liu, Y.,Abula, A.,Xiao, H.,Guo, H.,Li, T.,Zheng, L.,Chen, B.,Nguyen, H.C.,Ji, X.
Structural Insight Into hnRNP A2/B1 Homodimerization and DNA Recognition.
J.Mol.Biol., 435:167920-167920, 2023

PubMed Abstract: Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) has been identified as a nuclear DNA sensor. Upon viral infection, hnRNP A2/B1 recognizes pathogen-derived DNA as a homodimer, which is a prerequisite for its translocation to the cytoplasm to activate the interferon response. However, the DNA binding mechanism inducing hnRNP A2/B1 homodimerization is unknown. Here, we show the crystal structure of the RNA recognition motif (RRM) of hnRNP A2/B1 in complex with a U-shaped ssDNA, which mediates the formation of a newly observed protein dimer. Our biochemical assays and mutagenesis studies confirm that the hnRNP A2/B1 homodimer forms in solution by binding to pre-generated ssDNA or dsDNA with a U-shaped bulge. These results depict a potential functional state of hnRNP A2/B1 in antiviral immunity and other cellular processes.

PubMed: 36528084
DOI: 10.1016/j.jmb.2022.167920

Experimental method

X-RAY DIFFRACTION (2.644 Å)