8HNI
hnRNP A2/B1 RRMs in complex with telomeric DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 225.252, 98.306, 145.928 |
Unit cell angles | 90.00, 130.37, 90.00 |
Refinement procedure
Resolution | 46.801 - 2.644 |
R-factor | 0.2377 |
Rwork | 0.237 |
R-free | 0.26150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1q |
RMSD bond length | 0.003 |
RMSD bond angle | 0.524 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.690 |
High resolution limit [Å] | 2.640 | 2.640 |
Rpim | 0.089 | |
Number of reflections | 70959 | 3533 |
<I/σ(I)> | 32.6 | |
Completeness [%] | 99.7 | |
Redundancy | 5.6 | |
CC(1/2) | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 200 mM LiCl, 0.1 M MES pH 6.0 , 20% (w/v) polyethylene glycol (PEG) 6000 |