8HNI

hnRNP A2/B1 RRMs in complex with telomeric DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	225.252, 98.306, 145.928
Unit cell angles	90.00, 130.37, 90.00

Refinement procedure

Resolution	46.801 - 2.644
R-factor	0.2377
Rwork	0.237
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u1q
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2148: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.690
High resolution limit [Å]	2.640	2.640
Rpim		0.089
Number of reflections	70959	3533
<I/σ(I)>	32.6
Completeness [%]	99.7
Redundancy	5.6
CC(1/2)	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	200 mM LiCl, 0.1 M MES pH 6.0 , 20% (w/v) polyethylene glycol (PEG) 6000