8HIM
A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom
Summary for 8HIM
| Entry DOI | 10.2210/pdb8him/pdb |
| EMDB information | 34821 |
| Descriptor | DNA (34-MER), DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase subunit, ... (15 entities in total) |
| Functional Keywords | dna-dependent rna polymerase v, transcription |
| Biological source | Brassica oleracea (wild cabbage) More |
| Total number of polymer chains | 13 |
| Total formula weight | 518698.80 |
| Authors | |
| Primary citation | Xie, G.,Du, X.,Hu, H.,Li, S.,Cao, X.,Jacobsen, S.E.,Du, J. Structure and mechanism of the plant RNA polymerase V. Science, 379:1209-1213, 2023 Cited by PubMed Abstract: In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage, which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation. PubMed: 36893216DOI: 10.1126/science.adf8231 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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