8HIM
A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | T | DNA (34-MER) | polymer | 34 | 10482.8 | 1 | Brassica oleracea | ||
| 2 | N | DNA (34-MER) | polymer | 34 | 10463.7 | 1 | Brassica oleracea | ||
| 3 | P | RNA (5'-R(*UP*AP*UP*AP*UP*GP*CP*AP*UP*AP*AP*AP*GP*AP*CP*CP*AP*GP*GP*C)-3') | polymer | 20 | 6414.9 | 1 | Brassica oleracea | ||
| 4 | A | DNA-directed RNA polymerase V largest subunit | polymer | 2032 | 222876.9 | 1 | Brassica oleracea | ||
| 5 | C | RPOLD domain-containing protein | polymer | 319 | 35484.1 | 1 | UniProt (A0A0D3D418) Pfam (PF01193) Pfam (PF01000) In PDB | Brassica oleracea (wild cabbage) | |
| 6 | F | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 144 | 16686.3 | 1 | UniProt (A0A0D3BZZ8) Pfam (PF01192) In PDB | Brassica oleracea (wild cabbage) | |
| 7 | J | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 69 | 7982.3 | 1 | UniProt (A0A0D3AAT3) Pfam (PF01194) In PDB | Brassica oleracea (wild cabbage) | |
| 8 | K | RNA_pol_L_2 domain-containing protein | polymer | 116 | 13538.3 | 1 | UniProt (A0A0D3DS91) Pfam (PF13656) In PDB | Brassica oleracea (wild cabbage) | |
| 9 | L | DNA-directed RNA polymerases II, IV and V subunit 12 | polymer | 51 | 5983.8 | 1 | UniProt (A0A0D2ZPP3) Pfam (PF03604) In PDB | Brassica oleracea (wild cabbage) | |
| 10 | H | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 146 | 16607.3 | 1 | UniProt (A0A0D3DUD8) Pfam (PF03870) In PDB | Brassica oleracea (wild cabbage) | |
| 11 | I | DNA-directed RNA polymerase subunit | polymer | 114 | 13123.8 | 1 | UniProt (A0A3P6GD79) Pfam (PF02150) Pfam (PF01096) In PDB | Brassica oleracea (wild cabbage) | |
| 12 | E | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 230 | 26260.1 | 1 | Brassica oleracea (wild cabbage) | ||
| 13 | B | DNA-directed RNA polymerase IV and V subunit 2 | polymer | 1169 | 132377.8 | 1 | Brassica oleracea | ||
| 14 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 15 | A, C, J, L, I | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
C: 0 - 318 (UniProt: A0A0D3D418)
F: 1 - 144 (UniProt: A0A0D3BZZ8)
L: 1 - 51 (UniProt: A0A0D2ZPP3)
| PDB | External Database | Details |
|---|---|---|
| Thr 2 | Ser 3 | variant |
| PDB | External Database | Details |
|---|---|---|
| Glu 51 | Asp 51 | variant |
| PDB | External Database | Details |
|---|---|---|
| Glu 18 | Lys 18 | variant |
| Cys 32 | Arg 32 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 518282.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 416.8 | |
| All* | Total formula weight | 518698.8 |
