8H3W

Crystal structure of chicken egg lysozyme at ambient temperature

Summary for 8H3W

• Entry DOI: 10.2210/pdb8h3w/pdb
• Descriptor: Lysozyme C (2 entities in total)
• Functional Keywords: light source, structural biology, atomic resolution, structural dynamics, ambient temperature, turkish delight, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 14331.16

Authors

DeMirci, H. (deposition date: 2022-10-09, release date: 2023-03-22, Last modification date: 2024-10-23)

Primary citation

Gul, M.,Ayan, E.,Destan, E.,Johnson, J.A.,Shafiei, A.,Kepceoglu, A.,Yilmaz, M.,Ertem, F.B.,Yapici, I.,Tosun, B.,Baldir, N.,Tokay, N.,Nergiz, Z.,Karakadioglu, G.,Paydos, S.S.,Kulakman, C.,Ferah, C.K.,Guven, O.,Atalay, N.,Akcan, E.K.,Cetinok, H.,Arslan, N.E.,Sabanoglu, K.,Asci, B.,Tavli, S.,Gumusboga, H.,Altuntas, S.,Otsuka, M.,Fujita, M.,Teki N, S.,Ci Ftci, H.,Durdagi, S.,Karaca, E.,Kaplan Turkoz, B.,Kabasakal, B.V.,Kati, A.,DeMi Rci, H.
Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source.
Sci Rep, 13:8123-8123, 2023

PubMed Abstract: High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 Å and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.

PubMed: 37208392
DOI: 10.1038/s41598-023-33989-0

Experimental method

X-RAY DIFFRACTION (2.39 Å)