8H3W

Crystal structure of chicken egg lysozyme at ambient temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-08-30
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	79.030, 79.030, 38.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.940 - 2.390
R-factor	0.179
Rwork	0.173
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ijv
Data reduction software	CrysalisPro
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.940	2.450
High resolution limit [Å]	2.390	2.390
Rmerge	0.688	0.584
Number of reflections	5107	484
<I/σ(I)>	17.26
Completeness [%]	100.0
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.09 M HEPES-NaOH pH 7.5, 1.26 M sodium citrate tribasic dihydrate, 10% v/v glycerol